Multiplication et bulbaison in vitro du glaïeul (Gladiolus grandiflorus Hort.)

In vitro Multiplication and Bulb Formation of (Gladiolus grandiflorus Hort.). In order to set up a new bulb formation and micropropagation of gladiolus in vitro, trials were undertaken using two ornemental gladiolus cultivars 'Peter pears' and 'White friend ship'. The explants used are apical buds with 2 to 3 mm length taken from the cormes. In vitro initiation and multiplication were done for the two cultivars on Murashige and Skoog medium to which 2 mg. l-1 of BA and 0.5 mgl-1 of AIB were added. In vitro root development and bulb formation have been occurred in MS Agar Agar medium with 0.5 mg.l-1 of AIB 30 days later than the same MS medium containing 6% of saccharose and without Agar

A Deep Learning Approach Combining Auto-encoder with One-class SVM for DDoS Attack Detection in SDNs

Software Defined Networking (SDN) provides us with the capability of collecting network traffic information and managing networks proactively. Therefore, SDN facilitates the promotion of more robust and secure networks. Recently, several Machine Learning (ML)/Deep Learning (DL) intrusion detection approaches have been proposed to secure SDN networks. Currently, most of the proposed ML/DL intrusion detection approaches are based on supervised learning approach that required labelled and well-balanced datasets for training. However, this is time intensive and require significant human expertise to curate these datasets. These approaches cannot deal well with imbalanced and unlabeled datasets. In this paper, we propose a hybrid unsupervised DL approach using the stack autoencoder and One-class Support Vector Machine (SAE-1SVM) for Distributed Denial of Service (DDoS) attack detection. The experimental results show that the proposed algorithm can achieve an average accuracy of 99.35 % with a small set of flow features. The SAE-1SVM shows that it can reduce the processing time significantly while maintaining a high detection rate. In summary, the SAE-1SVM can work well with imbalanced and unlabeled datasets and yield a high detection accuracy

Towards the Formal Reliability Analysis of Oil and Gas Pipelines

It is customary to assess the reliability of underground oil and gas pipelines in the presence of excessive loading and corrosion effects to ensure a leak-free transport of hazardous materials. The main idea behind this reliability analysis is to model the given pipeline system as a Reliability Block Diagram (RBD) of segments such that the reliability of an individual pipeline segment can be represented by a random variable. Traditionally, computer simulation is used to perform this reliability analysis but it provides approximate results and requires an enormous amount of CPU time for attaining reasonable estimates. Due to its approximate nature, simulation is not very suitable for analyzing safety-critical systems like oil and gas pipelines, where even minor analysis flaws may result in catastrophic consequences. As an accurate alternative, we propose to use a higher-order-logic theorem prover (HOL) for the reliability analysis of pipelines. As a first step towards this idea, this paper provides a higher-order-logic formalization of reliability and the series RBD using the HOL theorem prover. For illustration, we present the formal analysis of a simple pipeline that can be modeled as a series RBD of segments with exponentially distributed failure times.Comment: 15 page

Formal Availability Analysis using Theorem Proving

Availability analysis is used to assess the possible failures and their restoration process for a given system. This analysis involves the calculation of instantaneous and steady-state availabilities of the individual system components and the usage of this information along with the commonly used availability modeling techniques, such as Availability Block Diagrams (ABD) and Fault Trees (FTs) to determine the system-level availability. Traditionally, availability analyses are conducted using paper-and-pencil methods and simulation tools but they cannot ascertain absolute correctness due to their inaccuracy limitations. As a complementary approach, we propose to use the higher-order-logic theorem prover HOL4 to conduct the availability analysis of safety-critical systems. For this purpose, we present a higher-order-logic formalization of instantaneous and steady-state availability, ABD configurations and generic unavailability FT gates. For illustration purposes, these formalizations are utilized to conduct formal availability analysis of a satellite solar array, which is used as the main source of power for the Dong Fang Hong-3 (DFH-3) satellite.Comment: 16 pages. arXiv admin note: text overlap with arXiv:1505.0264

High-Energy Molecular-Frame Photoelectron Angular Distributions: A Molecular Bond-Length Ruler

We present an experimental and theoretical study of core-level ionization of small hetero- and homo-nuclear molecules employing circularly polarized light and address molecular-frame photoelectron angular distributions in the light's polarization plane (CP-MFPADs). We find that the main forward-scattering peaks of CP-MFPADs are slightly tilted with respect to the molecular axis. We show that this tilt angle can be directly connected to the molecular bond length by a simple, universal formula. The extraction of the bond length becomes more accurate as the photoelectron energy is increased. We apply the derived formula to several examples of CP-MFPADs of C 1s and O 1s photoelectrons of CO, which have been measured experimentally or obtained by means of ab initio modeling. The photoelectron kinetic energies range from 70 to 1000~eV and the extracted bond lengths agree well with the known bond length of the CO molecule in its ground state. In addition, we discuss the influence of the back-scattering contribution that is superimposed over the analyzed forward-scattering peak in case of homo-nuclear diatomic molecules as N$_2$

Recent advances of metabolomics in plant biotechnology

Biotechnology, including genetic modification, is a very important approach to regulate the production of particular metabolites in plants to improve their adaptation to environmental stress, to improve food quality, and to increase crop yield. Unfortunately, these approaches do not necessarily lead to the expected results due to the highly complex mechanisms underlying metabolic regulation in plants. In this context, metabolomics plays a key role in plant molecular biotechnology, where plant cells are modified by the expression of engineered genes, because we can obtain information on the metabolic status of cells via a snapshot of their metabolome. Although metabolome analysis could be used to evaluate the effect of foreign genes and understand the metabolic state of cells, there is no single analytical method for metabolomics because of the wide range of chemicals synthesized in plants. Here, we describe the basic analytical advancements in plant metabolomics and bioinformatics and the application of metabolomics to the biological study of plants

Interaction of plant growth regulators and reactive oxygen species to regulate petal senescence in wallflowers (Erysimum linifolium)

Background In many species floral senescence is coordinated by ethylene. Endogenous levels rise, and exogenous application accelerates senescence. Furthermore, floral senescence is often associated with increased reactive oxygen species, and is delayed by exogenously applied cytokinin. However, how these processes are linked remains largely unresolved. Erysimum linifolium (wallflower) provides an excellent model for understanding these interactions due to its easily staged flowers and close taxonomic relationship to Arabidopsis. This has facilitated microarray analysis of gene expression during petal senescence and provided gene markers for following the effects of treatments on different regulatory pathways. Results In detached Erysimum linifolium (wallflower) flowers ethylene production peaks in open flowers. Furthermore senescence is delayed by treatments with the ethylene signalling inhibitor silver thiosulphate, and accelerated with ethylene released by 2-chloroethylphosphonic acid. Both treatments with exogenous cytokinin, or 6-methyl purine (which is an inhibitor of cytokinin oxidase), delay petal senescence. However, treatment with cytokinin also increases ethylene biosynthesis. Despite the similar effects on senescence, transcript abundance of gene markers is affected differentially by the treatments. A significant rise in transcript abundance of WLS73 (a putative aminocyclopropanecarboxylate oxidase) was abolished by cytokinin or 6-methyl purine treatments. In contrast, WFSAG12 transcript (a senescence marker) continued to accumulate significantly, albeit at a reduced rate. Silver thiosulphate suppressed the increase in transcript abundance both of WFSAG12 and WLS73. Activity of reactive oxygen species scavenging enzymes changed during senescence. Treatments that increased cytokinin levels, or inhibited ethylene action, reduced accumulation of hydrogen peroxide. Furthermore, although auxin levels rose with senescence, treatments that delayed early senescence did not affect transcript abundance of WPS46, an auxin-induced gene. Conclusions A model for the interaction between cytokinins, ethylene, reactive oxygen species and auxin in the regulation of floral senescence in wallflowers is proposed. The combined increase in ethylene and reduction in cytokinin triggers the initiation of senescence and these two plant growth regulators directly or indirectly result in increased reactive oxygen species levels. A fall in conjugated auxin and/or the total auxin pool eventually triggers abscission

Drought Impact Is Alleviated in Sugar Beets (Beta vulgaris L.) by Foliar Application of Fullerenol Nanoparticles

Over the past few years, significant efforts have been made to decrease the effects of drought stress on plant productivity and quality. We propose that fullerenol nanoparticles (FNPs, molecular formula C-60(OH)(24)) may help alleviate drought stress by serving as an additional intercellular water supply. Specifically, FNPs are able to penetrate plant leaf and root tissues, where they bind water in various cell compartments. This hydroscopic activity suggests that FNPs could be beneficial in plants. The aim of the present study was to analyse the influence of FNPs on sugar beet plants exposed to drought stress. Our results indicate that intracellular water metabolism can be modified by foliar application of FNPs in drought exposed plants. Drought stress induced a significant increase in the compatible osmolyte proline in both the leaves and roots of control plants, but not in FNP treated plants. These results indicate that FNPs could act as intracellular binders of water, creating an additional water reserve, and enabling adaptation to drought stress. Moreover, analysis of plant antioxidant enzyme activities (CAT, APx and GPx), MDA and GSH content indicate that fullerenol foliar application could have some beneficial effect on alleviating oxidative effects of drought stress, depending on the concentration of nanoparticles applied. Although further studies are necessary to elucidate the biochemical impact of FNPs on plants; the present results could directly impact agricultural practice, where available water supplies are often a limiting factor in plant bioproductivity